23CP

Cryo-EM structure of RSC complex from Chaetomium thermophilum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (K)	WD40 repeat-containing protein	polymer	1595	179692.9	1	UniProt (G0SHD8)	Thermochaetoides thermophila DSM 1495	Snf2
2	B, C (N, O)	Ssr1	polymer	694	75831.1	2	UniProt (G0S647)	Thermochaetoides thermophila DSM 1495
3	D (P)	DM2 domain-containing protein	polymer	547	61496.9	1	UniProt (G0RYN3)	Thermochaetoides thermophila DSM 1495	Ssr3
4	E (Q)	DUF1750-domain-containing protein	polymer	769	81947.3	1	UniProt (G0SEZ9)	Thermochaetoides thermophila DSM 1495	Ssr4
5	F (R)	Putative chromatin structure-remodeling complex protein	polymer	763	86517.7	1	UniProt (G0S909)	Thermochaetoides thermophila DSM 1495	Rsc9
6	G (S)	Putative chromatin structure-remodeling complex protein	polymer	607	66694.2	1	UniProt (G0S475)	Thermochaetoides thermophila DSM 1495	Sfh1
7	H (T)	Rsc7	polymer	534	60566.0	1	UniProt (G0S6N5)	Thermochaetoides thermophila DSM 1495
8	I (V)	Rsc58	polymer	990	107232.5	1	UniProt (G0S281)	Thermochaetoides thermophila DSM 1495
9	J (U)	Rsc1	polymer	991	112634.4	1	UniProt (G0SB54)	Thermochaetoides thermophila DSM 1495
10	K, L, M (N, O, S)	ZINC ION	non-polymer		65.4	3	Chemie (ZN) PubChem (32051)

Sequence modifications

K: 3 - 1536 (UniProt: G0SHD8)

PDB	External Database	Details
Gly -58	-	expression tag
Ser -57	-	expression tag
Pro -56	-	expression tag
Gln -55	-	expression tag
Gln -54	-	expression tag
Asn -53	-	expression tag
Lys -52	-	expression tag
Thr -51	-	expression tag
Ala -50	-	expression tag
Ala -49	-	expression tag
Leu -48	-	expression tag
Ala -47	-	expression tag
Gln -46	-	expression tag
His -45	-	expression tag
Asp -44	-	expression tag
Glu -43	-	expression tag
Ala -42	-	expression tag
Asp -41	-	expression tag
Tyr -40	-	expression tag
Lys -39	-	expression tag
Asp -38	-	expression tag
His -37	-	expression tag
Asp -36	-	expression tag
Gly -35	-	expression tag
Asp -34	-	expression tag
Tyr -33	-	expression tag
Lys -32	-	expression tag
Asp -31	-	expression tag
His -30	-	expression tag
Asp -29	-	expression tag
Ile -28	-	expression tag
Asp -27	-	expression tag
Tyr -26	-	expression tag
Lys -25	-	expression tag
Asp -24	-	expression tag
Asp -23	-	expression tag
Asp -22	-	expression tag
Asp -21	-	expression tag
Lys -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Gly -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Glu -12	-	expression tag
Val -11	-	expression tag
Leu -10	-	expression tag
Phe -9	-	expression tag
Gln -8	-	expression tag
Gly -7	-	expression tag
Pro -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Thr 2	-	expression tag
Val 114	Ile 891	conflict
Ile 282	Val 1059	conflict
Thr 1513	Ala 2290	conflict

N, O: 1 - 686 (UniProt: G0S647)

PDB	External Database	Details
Asp 687	-	expression tag
Tyr 688	-	expression tag
Lys 689	-	expression tag
Asp 690	-	expression tag
Asp 691	-	expression tag
Asp 692	-	expression tag
Asp 693	-	expression tag
Lys 694	-	expression tag

P: 1 - 539 (UniProt: G0RYN3)

PDB	External Database	Details
Asp 191	Asn 191	conflict
Asp 540	-	expression tag
Tyr 541	-	expression tag
Lys 542	-	expression tag
Asp 543	-	expression tag
Asp 544	-	expression tag
Asp 545	-	expression tag
Asp 546	-	expression tag
Lys 547	-	expression tag

Q: 1 - 762 (UniProt: G0SEZ9)

PDB	External Database	Details
Asp 763	-	expression tag
Tyr 764	-	expression tag
Lys 765	-	expression tag
Asp 766	-	expression tag
Asp 767	-	expression tag
Asp 768	-	expression tag
Asp 769	-	expression tag
Lys 770	-	expression tag

R: 270 - 1025 (UniProt: G0S909)

PDB	External Database	Details
Met 269	-	initiating methionine
Ile 444	Asn 444	conflict
Glu 601	Asp 601	conflict
Thr 741	Ser 741	conflict
His 1026	-	expression tag
His 1027	-	expression tag
His 1028	-	expression tag
His 1029	-	expression tag
His 1030	-	expression tag
His 1031	-	expression tag

S: 1 - 599 (UniProt: G0S475)

PDB	External Database	Details
Asp 6	Asn 6	conflict
Ile 272	Thr 272	conflict
Asp 600	-	expression tag
Tyr 601	-	expression tag
Lys 602	-	expression tag
Asp 603	-	expression tag
Asp 604	-	expression tag
Asp 605	-	expression tag
Asp 606	-	expression tag
Lys 607	-	expression tag

T: 1 - 526 (UniProt: G0S6N5)

PDB	External Database	Details
Asp 527	-	expression tag
Tyr 528	-	expression tag
Lys 529	-	expression tag
Asp 530	-	expression tag
Asp 531	-	expression tag
Asp 532	-	expression tag
Asp 533	-	expression tag
Lys 534	-	expression tag

V: 1 - 984 (UniProt: G0S281)

PDB	External Database	Details
Glu 300	Asp 300	conflict
His 612	Pro 612	conflict
His 985	-	expression tag
His 986	-	expression tag
His 987	-	expression tag
His 988	-	expression tag
His 989	-	expression tag
His 990	-	expression tag

U: 1 - 983 (UniProt: G0SB54)

PDB	External Database	Details
Gly 294	Asp 294	conflict
Asp 300	Asn 300	conflict
His 363	Tyr 363	conflict
Asp 984	-	expression tag
Tyr 985	-	expression tag
Lys 986	-	expression tag
Asp 987	-	expression tag
Asp 988	-	expression tag
Asp 989	-	expression tag
Asp 990	-	expression tag
Lys 991	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	908444.0
Non-Polymers*	Number of molecules	3
	Total formula weight	196.2
All*	Total formula weight	908640.2

*Water molecules are not included.