22YZ

Crystal structure of RipNE220A(82-474) from Ralstonia solanacearum

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Type III effector protein	polymer	400	43569.8	2	UniProt (Q8XQT8)	Ralstonia nicotianae (strain ATCC BAA-1114 / GMI1000) (Ralstonia solanacearum)	RipN
2	C, E (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	D (A)	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
4	F, G (A, B)	water	water		18.0	631	Chemie (HOH)

Sequence modifications

A, B: 83 - 474 (UniProt: Q8XQT8)

PDB	External Database	Details
Met 75	-	initiating methionine
Gly 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
His 81	-	expression tag
His 82	-	expression tag
Ala 220	Glu 220	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	87139.6
Non-Polymers*	Number of molecules	3
	Total formula weight	243.2
All*	Total formula weight	87382.8

*Water molecules are not included.