22VG

Cryo-EM structure of DOCK11 complexed with Cdc42(G15A) and Cdc42(Q61L)-GTP in the membrane-bound state

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	Dedicator of cytokinesis protein 11	polymer	2079	238396.0	2	UniProt (Q5JSL3)	Homo sapiens (human)	Activated Cdc42-associated guanine nucleotide exchange factor,ACG,Zizimin-2
2	B, E (C, D)	Cell division control protein 42 homolog	polymer	195	21475.6	2	UniProt (P60953)	Homo sapiens (human)	G25K GTP-binding protein
3	C, F (E, F)	Cell division control protein 42 homolog	polymer	195	21446.6	2	UniProt (P60953)	Homo sapiens (human)	G25K GTP-binding protein
4	G, I (E, F)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG) PubChem (888)
5	H, J (E, F)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
6	K, L (E, F)	water	water		18.0	4	Chemie (HOH)

Sequence modifications

A, B: 1 - 2073 (UniProt: Q5JSL3)

PDB	External Database	Details
Gly -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C, D: 1 - 187 (UniProt: P60953)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Ala 15	Gly 15	engineered mutation
Ser 188	-	expression tag

E, F: 1 - 187 (UniProt: P60953)

PDB	External Database	Details
Gly -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Leu 61	Gln 61	engineered mutation
Ser 188	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	562636.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1095.0
All*	Total formula weight	563731.3

*Water molecules are not included.