22TR

Cryo-EM structure of Crimean-Congo hemorrhagic fever virus RNA polymerase in complex with 5' vRNA promotor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-directed RNA polymerase L	polymer	3974	451547.6	1	UniProt (Q6TQR6)	Crimean-Congo hemorrhagic fever virus strain IbAr10200	Protein L,Large structural protein,Replicase,Transcriptase
2	B (B)	RNA (5'-R(P*UP*CP*UP*CP*AP*AP*AP*GP*AP*UP*AP*UP*CP*GP*U)-3')	polymer	15	4737.9	1		Crimean-Congo hemorrhagic fever virus strain IbAr10200
3	C, D (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN) PubChem (32051)
4	E (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN) PubChem (27854)

Sequence modifications

A: 1 - 3945 (UniProt: Q6TQR6)

PDB	External Database	Details
Trp 3946	-	expression tag
Ser 3947	-	expression tag
His 3948	-	expression tag
Pro 3949	-	expression tag
Gln 3950	-	expression tag
Phe 3951	-	expression tag
Glu 3952	-	expression tag
Lys 3953	-	expression tag
Gly 3954	-	expression tag
Ser 3955	-	expression tag
Ser 3956	-	expression tag
Gly 3957	-	expression tag
Gly 3958	-	expression tag
Ser 3959	-	expression tag
Ser 3960	-	expression tag
Gly 3961	-	expression tag
Ser 3962	-	expression tag
Ser 3963	-	expression tag
Gly 3964	-	expression tag
Gly 3965	-	expression tag
Ala 3966	-	expression tag
Trp 3967	-	expression tag
Ser 3968	-	expression tag
His 3969	-	expression tag
Pro 3970	-	expression tag
Gln 3971	-	expression tag
Phe 3972	-	expression tag
Glu 3973	-	expression tag
Lys 3974	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	456285.5
Non-Polymers*	Number of molecules	3
	Total formula weight	185.8
All*	Total formula weight	456471.2

*Water molecules are not included.