22PC

Crystal structure of the histone-binding motif of ATRX in complex with H3-H4-ASF1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, E)	Histone H3	polymer	84	9692.4	2	UniProt (A0A310TTQ1)	Xenopus laevis (African clawed frog)
2	B, E (B, F)	Histone H4	polymer	88	9820.5	2	UniProt (P62805)	Homo sapiens (human)
3	C, F (C, G)	Histone chaperone ASF1B	polymer	163	18326.6	2	UniProt (Q9NVP2)	Homo sapiens (human)	Anti-silencing function protein 1 homolog B,hAsf1,hAsf1b,CCG1-interacting factor A-II,CIA-II,hCIA-II
4	G, H (D, H)	Transcriptional regulator ATRX	polymer	23	2565.6	2	UniProt (P46100)	Homo sapiens (human)	ATP-dependent helicase ATRX,X-linked helicase II,X-linked nuclear protein,XNP,Znf-HX

Sequence modifications

A, E: 56 - 135 (UniProt: A0A310TTQ1)

PDB	External Database	Details
Met 52	-	initiating methionine
Ala 53	-	expression tag
Arg 54	-	expression tag
Ser 55	-	expression tag

B, F: 20 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly 15	-	expression tag
Pro 16	-	expression tag
Leu 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag

C, G: 1 - 158 (UniProt: Q9NVP2)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

D, H: 1900 - 1921 (UniProt: P46100)

PDB	External Database	Details
Ser 1899	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	80810.0
All*	Total formula weight	80810.0

*Water molecules are not included.