22OF

Cryo-EM structure of CeTECR-CeHACD complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable very-long-chain enoyl-CoA reductase art-1	polymer	309	35281.0	1	UniProt (Q9N5Y2) Pfam (PF21696) Pfam (PF02544)	Caenorhabditis elegans
2	B (B)	Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase hpo-8	polymer	241	27322.2	1	UniProt (O17040) Pfam (PF04387)	Caenorhabditis elegans	Probable 3-hydroxyacyl-CoA dehydratase
3	C (A)	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	non-polymer		745.4	1	Chemie (NDP)

Sequence modifications

A: 1 - 308 (UniProt: Q9N5Y2)

PDB	External Database	Details
Met 0	-	initiating methionine

B: 1 - 218 (UniProt: O17040)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Asp -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Asp -6	-	expression tag
Ala -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	62603.2
Non-Polymers*	Number of molecules	1
	Total formula weight	745.4
All*	Total formula weight	63348.6

*Water molecules are not included.