22FN
Cryo-EM structure of AsCas12a in complex with crDNA and RNA target
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | RNA (5'-R(P*GP*AP*CP*AP*GP*CP*CP*CP*AP*CP*AP*UP*GP*GP*CP*AP*UP*UP*CP*CP*AP*CP*U)-3') | polymer | 45 | 14179.4 | 1 | synthetic construct | ||
| 2 | B (A) | CRISPR-associated endonuclease Cas12a | polymer | 1307 | 151410.9 | 1 | UniProt (U2UMQ6) | Acidaminococcus sp. BV3L6 | AsCpf1,CRISPR-associated endonuclease Cpf1 |
| 3 | C (C) | DNA (41-MER) | polymer | 43 | 13352.6 | 1 | synthetic construct |
Sequence modifications
A: 1 - 1307 (UniProt: U2UMQ6)
| PDB | External Database | Details |
|---|---|---|
| Arg 537 | Met 537 | engineered mutation |
| Leu 870 | Phe 870 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 178943.0 | |
| All* | Total formula weight | 178943.0 |
