229L

GENERATING LIGAND BINDING SITES IN T4 LYSOZYME USING DEFICIENCY-CREATING SUBSTITUTIONS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	T4 LYSOZYME	polymer	164	18542.2	1	UniProt (P00720) Pfam (PF00959) In PDB	Enterobacteria phage T4
2	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
3	A	GUANIDINE	non-polymer		59.1	1	Chemie (GAI)
4	A	BETA-MERCAPTOETHANOL	non-polymer		78.1	2	Chemie (BME)
5		water	water		18.0	123	Chemie (HOH)

Sequence modifications

A: 1 - 164 (UniProt: P00720)

PDB	External Database	Details
Thr 54	Cys 54	engineered mutation
Ala 95	Arg 95	engineered mutation
Ala 97	Cys 97	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	18542.2
Non-Polymers*	Number of molecules	5
	Total formula weight	286.2
All*	Total formula weight	18828.5

*Water molecules are not included.