21WC

Cryo-EM structure of the ATPase domain of SMARCA4 and the finger helix of BCL7A bound to a nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3	polymer	135	15303.9	2	UniProt (A0A310TTQ1)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	125	13848.1	2	UniProt (A0A8J0U496)	Xenopus laevis (African clawed frog)
5	I (I)	SMARCA4 isoform 2	polymer	1711	191867.2	1	UniProt (Q9HBD4)	Homo sapiens (human)	SWI/SNF related,matrix associated,actin dependent regulator of chromatin,subfamily a,member 4
6	J (N)	B-cell CLL/lymphoma 7 protein family member A	polymer	216	23671.8	1	UniProt (Q4VC05)	Homo sapiens (human)
7	K (X)	DNA (167-MER)	polymer	167	51333.7	1		Homo sapiens (human)
8	L (Y)	DNA (167-MER)	polymer	167	51774.0	1		Homo sapiens (human)
9	M (I)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG) PubChem (888)
10	N (I)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
11	O (I)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	1	Chemie (BEF)

Sequence modifications

I: 1 - 1679 (UniProt: Q9HBD4)

PDB	External Database	Details
Gly -9	-	expression tag
Pro -8	-	expression tag
Gly -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag
Asp 1680	-	expression tag
Tyr 1681	-	expression tag
Lys 1682	-	expression tag
Asp 1683	-	expression tag
His 1684	-	expression tag
Asp 1685	-	expression tag
Gly 1686	-	expression tag
Asp 1687	-	expression tag
Tyr 1688	-	expression tag
Lys 1689	-	expression tag
Asp 1690	-	expression tag
His 1691	-	expression tag
Asp 1692	-	expression tag
Ile 1693	-	expression tag
Asp 1694	-	expression tag
Tyr 1695	-	expression tag
Lys 1696	-	expression tag
Asp 1697	-	expression tag
Asp 1698	-	expression tag
Asp 1699	-	expression tag
Asp 1700	-	expression tag
Lys 1701	-	expression tag

N: 1 - 210 (UniProt: Q4VC05)

PDB	External Database	Details
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
His 215	-	expression tag
His 216	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	427433.7
Non-Polymers*	Number of molecules	3
	Total formula weight	517.5
All*	Total formula weight	427951.2

*Water molecules are not included.