21TY

Cryo-EM structure of Free fatty acid receptor 2 (FFA2)-ARK1 with GLPG0974

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Free fatty acid receptor 2,human free fatty acid receptor 2 (FFA2) fused with de novo designed ARK1	polymer	748	85565.5	1	UniProt (O15552)	Homo sapiens (human)	G-protein coupled receptor 43
2	B (A)	GLPG-0974	non-polymer		485.0	1	Chemie (A1LYD) PubChem (57520598)
3	C (A)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A: 1 - 208 (UniProt: O15552)

PDB	External Database	Details
Met -30	-	initiating methionine
Lys -29	-	expression tag
Thr -28	-	expression tag
Ile -27	-	expression tag
Ile -26	-	expression tag
Ala -25	-	expression tag
Leu -24	-	expression tag
Ser -23	-	expression tag
Tyr -22	-	expression tag
Ile -21	-	expression tag
Phe -20	-	expression tag
Cys -19	-	expression tag
Leu -18	-	expression tag
Val -17	-	expression tag
Phe -16	-	expression tag
Ala -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Lys -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Ala -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

A: 209 - 596 (PDB: 21TY)
A: 597 - 711 (UniProt: O15552)

PDB	External Database	Details
Leu 712	-	expression tag
Glu 713	-	expression tag
Val 714	-	expression tag
Leu 715	-	expression tag
Phe 716	-	expression tag
Gln 717	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	85565.5
Non-Polymers*	Number of molecules	1
	Total formula weight	485.0
All*	Total formula weight	86050.4

*Water molecules are not included.