21TK

Enterovirus 71 Polymerase (S417G mutant) Elongation Complex with Two Remdesivir Nucleotides Posing between Product Strand Positions -4/-5 and -6/-7, respectively (C3S6 form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Enterovirus 71 polymerase	polymer	468	53378.4	1	UniProt (E5RPG3)	Enterovirus A71
2	B (B)	RNA (34-MER)	polymer	34	10954.6	1		synthetic construct
3	C (C)	RNA (5'-R(*UP*GP*UP*UP*CP*GP*AP*CP*GP*AP*G*(F86)P*G*(F86)P*GP*CP*CP*U)-3')	polymer	18	5836.5	1		synthetic construct
4	D (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN) PubChem (32051)
5	E, F (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
6	G, H, I (A, B, C)	water	water		18.0	431	Chemie (HOH)

Sequence modifications

A: 1 - 462 (UniProt: E5RPG3)

PDB	External Database	Details
Met 291	Cys 2022	engineered mutation
Gly 417	Ser 2148	engineered mutation
His 463	-	expression tag
His 464	-	expression tag
His 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	70169.5
Non-Polymers*	Number of molecules	3
	Total formula weight	257.5
All*	Total formula weight	70427.1

*Water molecules are not included.