21TJ
Enterovirus 71 Polymerase (S417G mutant) Elongation Complex with Two Remdesivir Nucleotides Posing between Product Strand Positions -4/-5 and -6/-7, respectively (C3S1o form)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Enterovirus 71 polymerase | polymer | 468 | 53378.4 | 1 | UniProt (E5RPG3) | Enterovirus A71 | |
| 2 | B (B) | RNA (34-MER) | polymer | 34 | 10954.6 | 1 | synthetic construct | ||
| 3 | C (C) | RNA (5'-R(*UP*GP*UP*UP*CP*GP*AP*CP*GP*AP*G*(F86)P*G*(F86)P*GP*CP*C)-3') | polymer | 17 | 5530.4 | 1 | synthetic construct | ||
| 4 | D (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) PubChem (32051) | |||
| 5 | E (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 6 | F (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 7 | G, H, I (A, B, C) | water | water | 18.0 | 269 | Chemie (HOH) |
Sequence modifications
A: 1 - 462 (UniProt: E5RPG3)
| PDB | External Database | Details |
|---|---|---|
| Met 291 | Cys 2022 | engineered mutation |
| Gly 417 | Ser 2148 | engineered mutation |
| His 463 | - | expression tag |
| His 464 | - | expression tag |
| His 465 | - | expression tag |
| His 466 | - | expression tag |
| His 467 | - | expression tag |
| His 468 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 69863.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 253.6 | |
| All* | Total formula weight | 70116.9 |
