21NZ

Cryo-EM structure of the rat IgE-Fc in complex with FcgammaRIV

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Low affinity immunoglobulin gamma Fc region receptor III-A	polymer	246	27381.6	1	UniProt (Q6XPU4)	Rattus norvegicus (Norway rat)	IgG Fc receptor III-A,CD16-2,FcgammaRIV
2	B, C (B, C)	Immunoglobulin heavy constant epsilon	polymer	363	41815.4	2	UniProt (P01855)	Rattus norvegicus (Norway rat)	Ig epsilon chain C region
3	D, F (D, F)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
4	E (E)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
5	G, H, I, J, K... (B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG) PubChem (24139)
6	M (C)	beta-D-mannopyranose	non-polymer		180.2	1	Chemie (BMA) PubChem (439680)

Sequence modifications

A: 1 - 203 (UniProt: Q6XPU4)

PDB	External Database	Details
Gly 204	-	expression tag
Gly 205	-	expression tag
Ser 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag
Gly 215	-	expression tag
Ser 216	-	expression tag
Ser 217	-	expression tag
Ala 218	-	expression tag
Trp 219	-	expression tag
Ser 220	-	expression tag
His 221	-	expression tag
Pro 222	-	expression tag
Gln 223	-	expression tag
Phe 224	-	expression tag
Glu 225	-	expression tag
Lys 226	-	expression tag
Gly 227	-	expression tag
Gly 228	-	expression tag
Gly 229	-	expression tag
Ser 230	-	expression tag
Gly 231	-	expression tag
Gly 232	-	expression tag
Gly 233	-	expression tag
Ser 234	-	expression tag
Gly 235	-	expression tag
Gly 236	-	expression tag
Ser 237	-	expression tag
Ala 238	-	expression tag
Trp 239	-	expression tag
Ser 240	-	expression tag
His 241	-	expression tag
Pro 242	-	expression tag
Gln 243	-	expression tag
Phe 244	-	expression tag
Glu 245	-	expression tag
Lys 246	-	expression tag

B, C: 95 - 429 (UniProt: P01855)

PDB	External Database	Details
Met 73	-	initiating methionine
Ser 74	-	expression tag
Val 75	-	expression tag
Pro 76	-	expression tag
Thr 77	-	expression tag
Gln 78	-	expression tag
Val 79	-	expression tag
Leu 80	-	expression tag
Gly 81	-	expression tag
Leu 82	-	expression tag
Leu 83	-	expression tag
Leu 84	-	expression tag
Leu 85	-	expression tag
Trp 86	-	expression tag
Leu 87	-	expression tag
Thr 88	-	expression tag
Asp 89	-	expression tag
Ala 90	-	expression tag
Arg 91	-	expression tag
Cys 92	-	expression tag
Asp 93	-	expression tag
Ile 94	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	111012.5
Branched	Number of molecules	3
	Total formula weight	1597.5
Non-Polymers*	Number of molecules	7
	Total formula weight	1507.4
All*	Total formula weight	114117.4

*Water molecules are not included.