21NY

Cryo-EM structure of the rat IgE-Fc in complex with FcgammaRIIB

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Low affinity immunoglobulin gamma Fc region receptor II	polymer	255	28627.2	1		Rattus norvegicus (Norway rat)
2	B, C (B, C)	Immunoglobulin heavy constant epsilon	polymer	363	41815.4	2	UniProt (P01855)	Rattus norvegicus (Norway rat)	Ig epsilon chain C region
3	D, E (D, E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
4	F, G, I, J, K... (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	9	Chemie (NAG) PubChem (24139)
5	H (A)	beta-D-mannopyranose	non-polymer		180.2	1	Chemie (BMA) PubChem (439680)

Sequence modifications

B, C: 95 - 429 (UniProt: P01855)

PDB	External Database	Details
Met 73	-	initiating methionine
Ser 74	-	expression tag
Val 75	-	expression tag
Pro 76	-	expression tag
Thr 77	-	expression tag
Gln 78	-	expression tag
Val 79	-	expression tag
Leu 80	-	expression tag
Gly 81	-	expression tag
Leu 82	-	expression tag
Leu 83	-	expression tag
Leu 84	-	expression tag
Leu 85	-	expression tag
Trp 86	-	expression tag
Leu 87	-	expression tag
Thr 88	-	expression tag
Asp 89	-	expression tag
Ala 90	-	expression tag
Arg 91	-	expression tag
Cys 92	-	expression tag
Asp 93	-	expression tag
Ile 94	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	112258.0
Branched	Number of molecules	2
	Total formula weight	1173.1
Non-Polymers*	Number of molecules	10
	Total formula weight	2171.0
All*	Total formula weight	115602.2

*Water molecules are not included.