21ME

Cryo-EM structure of heterotypic CENP-A/H3 nucleosome isolated from human cells

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Histone H3-like centromeric protein A	polymer	162	18639.5	1	UniProt (P49450)	Homo sapiens (human)	Centromere autoantigen A,Centromere protein A,CENP-A
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62805)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	130	14165.6	2	UniProt (P04908)	Homo sapiens (human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (D, H)	Histone H2B type 1-J	polymer	126	13935.2	2	UniProt (P06899)	Homo sapiens (human)	Histone H2B.1,Histone H2B.r,H2B/r
5	E (E)	Histone H3.1	polymer	136	15437.2	1	UniProt (P68431)	Homo sapiens (human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
6	I, J (I, J)	DNA (121-MER)	polymer	121	21746.7	2		Homo sapiens

Sequence modifications

A: 1 - 140 (UniProt: P49450)

PDB	External Database	Details
Gly 141	-	expression tag
Arg 142	-	expression tag
Ile 143	-	expression tag
Leu 144	-	expression tag
Glu 145	-	expression tag
Val 146	-	expression tag
Leu 147	-	expression tag
Phe 148	-	expression tag
Gln 149	-	expression tag
Gly 150	-	expression tag
Pro 151	-	expression tag
His 152	-	expression tag
Met 153	-	expression tag
Asp 154	-	expression tag
Tyr 155	-	expression tag
Lys 156	-	expression tag
Asp 157	-	expression tag
Asp 158	-	expression tag
Asp 159	-	expression tag
Asp 160	-	expression tag
Lys 161	-	expression tag
His 162	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	156560.4
All*	Total formula weight	156560.4

*Water molecules are not included.