21KS

A Wnt3a/Fzd8-CRD/LRP6-E3E4-LA complex with FKBP

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, G (C, G, D, H, I)	Frizzled-8,Peptidyl-prolyl cis-trans isomerase FKBP1A	polymer	271	29934.6	5	UniProt (Q9H461) UniProt (P62942)	Homo sapiens (human)	Fz-8,hFz8,PPIase FKBP1A,12 kDa FK506-binding protein,12 kDa FKBP,FKBP-12,Calstabin-1,FK506-binding protein 1A,FKBP-1A,Immunophilin FKBP12,Rotamase
2	E, H (A, E)	Protein Wnt-3a	polymer	334	37438.3	2	UniProt (P27467)	Mus musculus (house mouse)
3	F, I (B, F)	Low-density lipoprotein receptor-related protein 6,Serine/threonine-protein kinase mTOR	polymer	877	98974.9	2	UniProt (O75581) UniProt (A0A8V8TRG9)	Homo sapiens (human)	LRP-6
4	J, K, L, M (J, K, L, M)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
5	N, O (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG) PubChem (24139)
6	P (I)	RAPAMYCIN IMMUNOSUPPRESSANT DRUG	non-polymer		914.2	1	Chemie (RAP) PubChem (5284616)

Sequence modifications

C, G, D, H, I: 31 - 155 (UniProt: Q9H461)

PDB	External Database	Details
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Ala 28	-	expression tag
Ser 29	-	expression tag
Ala 30	-	expression tag
Gly 156	-	linker
Gly 157	-	linker
Gly 158	-	linker
Gly 159	-	linker
Ser 160	-	linker
Gly 161	-	linker
Gly 162	-	linker
Gly 163	-	linker
Gly 164	-	linker
Ser 165	-	linker
Gly 166	-	linker
Gly 167	-	linker
Gly 168	-	linker
Gly 169	-	linker
Ser 170	-	linker
Gly 171	-	linker
Gly 172	-	linker
Gly 173	-	linker
Gly 174	-	linker
Ser 175	-	linker

C, G, D, H, I: 176 - 282 (UniProt: P62942)

PDB	External Database	Details
Ser 197	Cys 23	engineered mutation
Asp 283	-	expression tag
Tyr 284	-	expression tag
Lys 285	-	expression tag
Asp 286	-	expression tag
Asp 287	-	expression tag
Asp 288	-	expression tag
Asp 289	-	expression tag
Lys 290	-	expression tag

B, F: 630 - 1370 (UniProt: O75581)

PDB	External Database	Details
Asp 622	-	expression tag
Tyr 623	-	expression tag
Lys 624	-	expression tag
Asp 625	-	expression tag
Asp 626	-	expression tag
Asp 627	-	expression tag
Asp 628	-	expression tag
Lys 629	-	expression tag
Gly 1371	-	linker
Gly 1372	-	linker
Gly 1373	-	linker
Gly 1374	-	linker
Ser 1375	-	linker
Gly 1376	-	linker
Gly 1377	-	linker
Gly 1378	-	linker
Gly 1379	-	linker
Ser 1380	-	linker
Gly 1381	-	linker
Gly 1382	-	linker
Gly 1383	-	linker
Gly 1384	-	linker
Ser 1385	-	linker
Gly 1386	-	linker
Gly 1387	-	linker
Gly 1388	-	linker
Gly 1389	-	linker
Ser 1390	-	linker

B, F: 1391 - 1490 (UniProt: A0A8V8TRG9)

PDB	External Database	Details
Ser 1461	Cys 2014	engineered mutation
His 1491	-	expression tag
His 1492	-	expression tag
His 1493	-	expression tag
His 1494	-	expression tag
His 1495	-	expression tag
His 1496	-	expression tag
His 1497	-	expression tag
His 1498	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	422499.6
Branched	Number of molecules	4
	Total formula weight	1697.6
Non-Polymers*	Number of molecules	3
	Total formula weight	1356.6
All*	Total formula weight	425553.8

*Water molecules are not included.