21DH

Crystal structure of MBP-fused Monobody P' in complex with HPPU

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Maltose/maltodextrin-binding periplasmic protein,Monobody P'	polymer	487	53901.5	6	UniProt (P0AEX9) Pfam (PF01547)	Escherichia coli K-12	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
2	G, H, I, J, K... (G, H, I, J, K...)	alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		342.3	6	In PDB BIRD (PRD_900018) GlyTouCan (G66120GK)
3	M, N, O, P, Q... (A, B, C, D, E...)	1-(4-hydroxyphenyl)-3-phenyl-urea	non-polymer		228.2	6	Chemie (A1MC1)
4	S, T, U, V, W... (A, B, C, D, E...)	water	water		18.0	198	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 17 - 378 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
Lys 13	-	expression tag
Ile 14	-	expression tag
Glu 15	-	expression tag
Glu 16	-	expression tag
Val 324	Ala 338	engineered mutation

A, B, C, D, E, F: 379 - 487 (PDB: 21DH)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	323409.0
Branched	Number of molecules	6
	Total formula weight	2053.8
Non-Polymers*	Number of molecules	6
	Total formula weight	1369.5
All*	Total formula weight	326832.2

*Water molecules are not included.