21CQ
Cryo-EM structure of DICER-D991G-H992G/26S-GU complex in dicing state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | RNA (64-MER) | polymer | 64 | 20545.3 | 1 | synthetic construct | ||
| 2 | B (D) | Endoribonuclease Dicer | polymer | 1909 | 217849.9 | 1 | UniProt (Q9UPY3) Pfam (PF00270) Pfam (PF20930) Pfam (PF00271) Pfam (PF03368) Pfam (PF20931) Pfam (PF02170) Pfam (PF00636) Pfam (PF20932) | Homo sapiens (human) | Helicase with RNase motif,Helicase MOI |
| 3 | C, D (R, D) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) |
Sequence modifications
D: 26 - 1922 (UniProt: Q9UPY3)
| PDB | External Database | Details |
|---|---|---|
| Met 14 | - | initiating methionine |
| Gly 15 | - | expression tag |
| His 16 | - | expression tag |
| His 17 | - | expression tag |
| His 18 | - | expression tag |
| His 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| His 22 | - | expression tag |
| His 23 | - | expression tag |
| His 24 | - | expression tag |
| His 25 | - | expression tag |
| Gly 991 | Asp 991 | engineered mutation |
| Gly 992 | His 992 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 238395.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 80.2 | |
| All* | Total formula weight | 238475.3 |
