20ZK
Cryo-EM structure of the human neurotensin receptor 1 (hNTSR1)-Gi1 complex in the GTP-bound, AHD-open NC state 3, plunge-frozen 0-5 seconds after GTP addition
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 358 | 39418.1 | 1 | UniProt (P62873) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) | Homo sapiens (human) | G gamma-I |
| 4 | D (D) | scFv16 | polymer | 259 | 27784.9 | 1 | Mus musculus | ||
| 5 | E (R) | Neurotensin receptor type 1 | polymer | 436 | 48535.1 | 1 | UniProt (P30989) | Homo sapiens (human) | NT-R-1,NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH |
| 6 | F (L) | LYS-LYS-PRO-TYR-ILE-LEU | polymer | 6 | 763.0 | 1 | synthetic construct |
Sequence modifications
B: 20 - 358 (UniProt: P62873)
R: 32 - 430 (UniProt: P30989)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| His 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| Leu 8 | - | expression tag |
| Glu 9 | - | expression tag |
| Val 10 | - | expression tag |
| Leu 11 | - | expression tag |
| Phe 12 | - | expression tag |
| Gln 13 | - | expression tag |
| Gly 14 | - | expression tag |
| Pro 15 | - | expression tag |
| Gly 16 | - | expression tag |
| Ser 17 | - | expression tag |
| Ser 18 | - | expression tag |
| Gly 19 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| Gln 3 | - | expression tag |
| Pro 4 | - | expression tag |
| Gly 5 | - | expression tag |
| Asn 6 | - | expression tag |
| Gly 7 | - | expression tag |
| Ser 8 | - | expression tag |
| Ala 9 | - | expression tag |
| Phe 10 | - | expression tag |
| Leu 11 | - | expression tag |
| Leu 12 | - | expression tag |
| Ala 13 | - | expression tag |
| Pro 14 | - | expression tag |
| Asn 15 | - | expression tag |
| Arg 16 | - | expression tag |
| Ser 17 | - | expression tag |
| His 18 | - | expression tag |
| Ala 19 | - | expression tag |
| Pro 20 | - | expression tag |
| Asp 21 | - | expression tag |
| His 22 | - | expression tag |
| Asp 23 | - | expression tag |
| Val 24 | - | expression tag |
| Glu 25 | - | expression tag |
| Asn 26 | - | expression tag |
| Leu 27 | - | expression tag |
| Tyr 28 | - | expression tag |
| Phe 29 | - | expression tag |
| Gln 30 | - | expression tag |
| Gly 31 | - | expression tag |
| Leu 97 | Ala 85 | engineered mutation |
| Leu 431 | - | expression tag |
| Glu 432 | - | expression tag |
| Val 433 | - | expression tag |
| Leu 434 | - | expression tag |
| Phe 435 | - | expression tag |
| Gln 436 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 164777.2 | |
| All* | Total formula weight | 164777.2 |
