20ZD

Cryo-EM structure of human Neurotensin Receptor 1 (hNTSR1)-Gi1 (delipidated) complex in nucleotide-free NC state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40399.0	1	UniProt (P63096)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (R)	Neurotensin receptor type 1	polymer	436	48535.1	1	UniProt (P30989)	Homo sapiens (human)	NT-R-1,NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH
5	E (L)	LYS-LYS-PRO-TYR-ILE-LEU	polymer	6	763.0	1		synthetic construct

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Ser 3	Cys 3	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

C: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Ser 68	Cys 68	engineered mutation

R: 20 - 418 (UniProt: P30989)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
Gln -9	-	expression tag
Pro -8	-	expression tag
Gly -7	-	expression tag
Asn -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Ala -3	-	expression tag
Phe -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Ala 1	-	expression tag
Pro 2	-	expression tag
Asn 3	-	expression tag
Arg 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
Ala 7	-	expression tag
Pro 8	-	expression tag
Asp 9	-	expression tag
His 10	-	expression tag
Asp 11	-	expression tag
Val 12	-	expression tag
Glu 13	-	expression tag
Asn 14	-	expression tag
Leu 15	-	expression tag
Tyr 16	-	expression tag
Phe 17	-	expression tag
Gln 18	-	expression tag
Gly 19	-	expression tag
Leu 85	Ala 85	engineered mutation
Leu 419	-	expression tag
Glu 420	-	expression tag
Val 421	-	expression tag
Leu 422	-	expression tag
Phe 423	-	expression tag
Gln 424	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	136960.2
All*	Total formula weight	136960.2

*Water molecules are not included.