1ZXE

Crystal Structure of eIF2alpha Protein Kinase GCN2: D835N Inactivating Mutant in Apo Form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Serine/threonine-protein kinase	polymer	303	35564.5	6	UniProt (P15442) Pfam (PF00069)	Saccharomyces cerevisiae (baker's yeast)
2	G, H, I, J, K... (A, B, C, D, E...)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
3	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	279	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 594 - 664 (UniProt: P15442)

PDB	External Database	Details
Ser 592	-	cloning artifact
Leu 593	-	cloning artifact
Mse 645	Met 576	modified residue

A, B, C, D, E, F: 768 - 997 (UniProt: P15442)

PDB	External Database	Details
Mse 775	Met 706	modified residue
Mse 788	Met 719	modified residue
Asn 835	Asp 766	engineered mutation
Mse 839	Met 770	modified residue
Mse 889	Met 820	modified residue
Mse 908	Met 839	modified residue
Mse 918	Met 849	modified residue
Mse 926	Met 857	modified residue
Mse 951	Met 882	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	213387.3
Non-Polymers*	Number of molecules	6
	Total formula weight	552.6
All*	Total formula weight	213939.8

*Water molecules are not included.