1ZP4
Glu28Gln mutant of E. coli Methylenetetrahydrofolate Reductase (oxidized) complex with Methyltetrahydrofolate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | 5,10-methylenetetrahydrofolate reductase | polymer | 304 | 34214.9 | 3 | UniProt (P00394) Pfam (PF02219) UniProt (by SIFTS) (P0AEZ1) | Escherichia coli | |
| 2 | D, I (A, C) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 3 | E, G, J (A, B, C) | 5-METHYL-5,6,7,8-TETRAHYDROFOLIC ACID | non-polymer | 459.5 | 3 | Chemie (C2F) | |||
| 4 | F, H, K (A, B, C) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 3 | Chemie (FAD) | |||
| 5 | L, M, N (A, B, C) | water | water | 18.0 | 509 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 296 (UniProt: P00394)
| PDB | External Database | Details |
|---|---|---|
| Gln 28 | Glu 28 | engineered mutation |
| Leu 297 | - | cloning artifact |
| Glu 298 | - | cloning artifact |
| His 299 | - | expression tag |
| His 300 | - | expression tag |
| His 301 | - | expression tag |
| His 302 | - | expression tag |
| His 303 | - | expression tag |
| His 304 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 102644.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 3927.1 | |
| All* | Total formula weight | 106571.7 |
