1YZW

The 2.1A Crystal Structure of the Far-red Fluorescent Protein HcRed: Inherent Conformational Flexibility of the Chromophore

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	GFP-like non-fluorescent chromoprotein	polymer	227	25650.2	4	UniProt (Q95W85) Pfam (PF01353)	Heteractis crispa (leathery sea anemone)	HcRed
2	E, F (B, C)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
3	G, H, I, J (A, B, C, D)	water	water		18.0	440	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 228 (UniProt: Q95W85)

PDB	External Database	Details
Ala 37	Thr 36	SEE REMARK 999
Cru 64	Glu 63	chromophore
Cru 64	Tyr 64	chromophore
Cru 64	Gly 65	chromophore
His 123	Leu 122	SEE REMARK 999
Ser 144	Cys 143	SEE REMARK 999
His 169	Arg 168	SEE REMARK 999
His 174	Leu 173	SEE REMARK 999
Leu 202	Pro 201	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	102600.7
Non-Polymers*	Number of molecules	2
	Total formula weight	212.2
All*	Total formula weight	102812.9

*Water molecules are not included.