1YSJ

Crystal Structure of Bacillus Subtilis YXEP Protein (APC1829), a Dinuclear Metal Binding Peptidase from M20 Family

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	protein yxeP	polymer	404	44842.0	2	UniProt (P54955) Pfam (PF01546) Pfam (PF07687)	Bacillus subtilis
2	C, D, E, F (A, B)	NICKEL (II) ION	non-polymer		58.7	4	Chemie (NI)
3	G, H (A, B)	water	water		18.0	312	Chemie (HOH)

Sequence modifications

A, B: 1 - 380 (UniProt: P54955)

PDB	External Database	Details
Mse -23	-	initiating methionine
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	cloning artifact
Ser -15	-	cloning artifact
Gly -14	-	cloning artifact
Val -13	-	cloning artifact
Asp -12	-	cloning artifact
Leu -11	-	cloning artifact
Gly -10	-	cloning artifact
Thr -9	-	cloning artifact
Glu -8	-	cloning artifact
Asn -7	-	cloning artifact
Leu -6	-	cloning artifact
Tyr -5	-	cloning artifact
Phe -4	-	cloning artifact
Gln -3	-	cloning artifact
Ser -2	-	cloning artifact
Asn -1	-	cloning artifact
Ala 0	-	cloning artifact
Mse 1	Met 1	modified residue
Mse 13	Met 13	modified residue
Gly 54	Ala 54	engineered mutation
Mse 95	Met 95	modified residue
Mse 112	Met 112	modified residue
Mse 157	Met 157	modified residue
Mse 176	Met 176	modified residue
Mse 249	Met 249	modified residue
Mse 268	Met 268	modified residue
Mse 341	Met 341	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	89683.9
Non-Polymers*	Number of molecules	4
	Total formula weight	234.8
All*	Total formula weight	89918.7

*Water molecules are not included.