1YRO

Crystal structure of beta14,-galactosyltransferase mutant ARG228Lys in complex with alpha-lactalbumin in the presence of UDP-galactose and Mn

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	ALPHA-LACTALBUMIN	polymer	123	14015.8	2	UniProt (P29752) Pfam (PF00062)	Mus musculus (house mouse)	LACTALBUMIN, ALPHA
2	B, D (B, D)	BETA-1,4-GALACTOSYLTRANSFERASE	polymer	286	32821.6	2	UniProt (P08037) Pfam (PF13733) Pfam (PF02709)	Bos taurus (cattle)	B4GAL-T1; BETA4GAL-T1; BETA-1,4-GALTASE 1; BETA-1,4-GALACTOSYLTRANSFERASE 1; UDP-GALACTOSE:BETA-N-ACETYLGLUCOSAMINE BETA-1,4-GALACTOSYLTRANSFERASE 1
3	E, K (A, C)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	F (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
5	G, L (A, C)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
6	H, M (B, D)	GALACTOSE-URIDINE-5'-DIPHOSPHATE	non-polymer		566.3	2	Chemie (GDU)
7	I, N (B, D)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
8	J, O (B, D)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
9	P, Q, R, S (A, B, C, D)	water	water		18.0	830	Chemie (HOH)

Sequence modifications

B, D: 130 - 402 (UniProt: P08037)

PDB	External Database	Details
Ala 117	-	SEE REMARK 999
Ser 118	-	SEE REMARK 999
Met 119	-	SEE REMARK 999
Thr 120	-	SEE REMARK 999
Gly 121	-	SEE REMARK 999
Gly 122	-	SEE REMARK 999
Gln 123	-	SEE REMARK 999
Gln 124	-	SEE REMARK 999
Met 125	-	SEE REMARK 999
Gly 126	-	SEE REMARK 999
Arg 127	-	SEE REMARK 999
Gly 128	-	SEE REMARK 999
Ser 129	-	SEE REMARK 999
Lys 228	Arg 155	engineered mutation
Thr 342	Cys 269	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	93674.8
Non-Polymers*	Number of molecules	11
	Total formula weight	2714.6
All*	Total formula weight	96389.4

*Water molecules are not included.