1YLH

Crystal Structure of Phosphoenolpyruvate Carboxykinase from Actinobaccilus succinogenes in Complex with Manganese and Pyruvate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	phosphoenolpyruvate carboxykinase	polymer	560	61895.7	1	UniProt (Q6W6X5) Pfam (PF01293) UniProt (by SIFTS) (A6VKV4) In PDB	Actinobacillus succinogenes
2	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
3	A	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	A	(2S,3S)-2,3-DIHYDROXY-4-SULFANYLBUTANE-1-SULFONATE	non-polymer		201.2	1	Chemie (DT3)
5	A	BETA-MERCAPTOETHANOL	non-polymer		78.1	2	Chemie (BME)
6	A	PYRUVIC ACID	non-polymer		88.1	1	Chemie (PYR)
7	A	FORMIC ACID	non-polymer		46.0	3	Chemie (FMT)
8		water	water		18.0	197	Chemie (HOH)

Sequence modifications

A: 2 - 540 (UniProt: Q6W6X5)

PDB	External Database	Details
Met -20	-	cloning artifact
Gly -19	-	cloning artifact
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Asp -12	-	cloning artifact
Tyr -11	-	cloning artifact
Asp -10	-	cloning artifact
Ile -9	-	cloning artifact
Pro -8	-	cloning artifact
Thr -7	-	cloning artifact
Thr -6	-	cloning artifact
Glu -5	-	cloning artifact
Asn -4	-	cloning artifact
Leu -3	-	cloning artifact
Tyr -2	-	cloning artifact
Phe -1	-	cloning artifact
Gln 0	-	cloning artifact
Gly 1	-	cloning artifact

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	61895.7
Non-Polymers*	Number of molecules	10
	Total formula weight	828.5
All*	Total formula weight	62724.2

*Water molecules are not included.