1YKH

Structure of the mediator MED7/MED21 (Med7/Srb7) subcomplex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RNA polymerase II mediator complex protein MED7	polymer	108	12964.0	1	UniProt (Q08278) Pfam (PF05983) In PDB	Saccharomyces cerevisiae (baker's yeast)	RNA polymerase II transcriptional regulation mediator 7
2	B	RNA polymerase II holoenzyme component SRB7	polymer	132	15315.5	1	UniProt (P47822) Pfam (PF11221) In PDB	Saccharomyces cerevisiae (baker's yeast)	MED21, RNAPII complex component SRB7, Suppressor of RNA polymerase B SRB7

Sequence modifications

A: 102 - 205 (UniProt: Q08278)

PDB	External Database	Details
Met 98	-	expression tag
Ala 99	-	expression tag
Ser 100	-	expression tag
Met 101	-	expression tag

B: 1 - 132 (UniProt: P47822)

PDB	External Database	Details
Met 5	Leu 5	engineered mutation
Met 119	Leu 119	engineered mutation
Met 125	Leu 125	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	28279.5
All*	Total formula weight	28279.5

*Water molecules are not included.