1YKE

Structure of the mediator MED7/MED21 subcomplex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	RNA polymerase II mediator complex protein MED7	polymer	108	12964.0	2	UniProt (Q08278) Pfam (PF05983) In PDB	Saccharomyces cerevisiae (baker's yeast)	RNA polymerase II transcriptional regulation mediator 7
2	B, D	RNA polymerase II holoenzyme component SRB7	polymer	151	17377.8	2	UniProt (P47822) Pfam (PF11221) In PDB	Saccharomyces cerevisiae (baker's yeast)	MED21, RNAPII complex component SRB7, Suppressor of RNA polymerase B SRB7

Sequence modifications

A, C: 102 - 205 (UniProt: Q08278)

PDB	External Database	Details
Met 98	-	cloning artifact
Ala 99	-	cloning artifact
Ser 100	-	cloning artifact
Met 101	-	cloning artifact

B, D: 1 - 140 (UniProt: P47822)

PDB	External Database	Details
Ala 141	-	expression tag
Ala 142	-	expression tag
Ala 143	-	expression tag
Leu 144	-	expression tag
Glu 145	-	expression tag
His 146	-	expression tag
His 147	-	expression tag
His 148	-	expression tag
His 149	-	expression tag
His 150	-	expression tag
His 151	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	60683.5
All*	Total formula weight	60683.5

*Water molecules are not included.