1YJW

Crystal Structure Of Quinupristin Bound To The G2099A Mutant 50S Ribosomal Subunit Of Haloarcula Marismortui

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (0)	23S RIBOSOMAL RNA	polymer	2922	946141.4	1	GenBank (55229667)	Haloarcula marismortui
2	B (1)	50S ribosomal protein L37e	polymer	57	6330.2	1	UniProt (P32410) Pfam (PF01907)	Haloarcula marismortui	L35e
3	C (2)	50S ribosomal protein L39e	polymer	50	6133.1	1	UniProt (P22452) Pfam (PF00832)	Haloarcula marismortui	Hl39e,Hl46e
4	D (3)	50S ribosomal protein L44e	polymer	92	10815.2	1	UniProt (P32411) Pfam (PF00935)	Haloarcula marismortui	HLA,La
5	E (4)	QUINUPRISTIN	polymer	8	1024.2	1		Streptomyces graminofaciens
6	F (9)	5S RIBOSOMAL RNA	polymer	122	39303.4	1	GenBank (43619)	Haloarcula marismortui
7	G (A)	50S ribosomal protein L2	polymer	240	25354.7	1	UniProt (P20276) Pfam (PF00181) Pfam (PF03947)	Haloarcula marismortui	Hl4,Hmal2
8	H (B)	50S ribosomal protein L3	polymer	338	37396.4	1	UniProt (P20279) Pfam (PF00297)	Haloarcula marismortui	Hl1,Hmal3
9	I (C)	50S ribosomal protein L4	polymer	246	26442.1	1	UniProt (P12735) Pfam (PF00573)	Haloarcula marismortui	Hl6,Hmal4
10	J (D)	50S ribosomal protein L5	polymer	177	19551.7	1	UniProt (P14124) Pfam (PF00281) Pfam (PF00673)	Haloarcula marismortui	Hl13,Hmal5
11	K (E)	50S ribosomal protein L6	polymer	178	19961.7	1	UniProt (P14135) Pfam (PF00347)	Haloarcula marismortui	Hl10,Hmal6
12	L (F)	50S ribosomal protein L7Ae	polymer	120	12791.9	1	UniProt (P12743) Pfam (PF01248)	Haloarcula marismortui	Hs6,Ribosomal protein L8e
13	M (G)	50S ribosomal protein L10	polymer	348	37213.8	1	UniProt (P15825) Pfam (PF00466) Pfam (PF17777)	Haloarcula marismortui	Acidic ribosomal protein P0 homolog,HMal10,L10E
14	N (H)	50S ribosomal protein L10e	polymer	177	19942.7	1	UniProt (P60617) Pfam (PF00252)	Haloarcula marismortui
15	O (I)	50S ribosomal protein L11	polymer	162	17097.5	1	UniProt (P14122) Pfam (PF03946) Pfam (PF00298)	Haloarcula marismortui	Hmal11
16	P (J)	50S ribosomal protein L13	polymer	145	16249.2	1	UniProt (P29198) Pfam (PF00572)	Haloarcula marismortui	Hmal13
17	Q (K)	50S ribosomal protein L14	polymer	132	14191.2	1	UniProt (P22450) Pfam (PF00238)	Haloarcula marismortui	Hl27,Hmal14
18	R (L)	50S ribosomal protein L15	polymer	165	18005.4	1	UniProt (P12737) Pfam (PF00828)	Haloarcula marismortui	Hl9,Hmal15
19	S (M)	50S ribosomal protein L15e	polymer	195	22275.1	1	UniProt (P60618) Pfam (PF00827)	Haloarcula marismortui	50S ribosomal protein LC12
20	T (N)	50S ribosomal protein L18	polymer	187	20640.9	1	UniProt (P14123) Pfam (PF17144)	Haloarcula marismortui	Hl12,Hmal18
21	U (O)	50S ribosomal protein L18e	polymer	116	12438.9	1	UniProt (P12733) Pfam (PF00828)	Haloarcula marismortui	Hl29,L19
22	V (P)	50S ribosomal protein L19e	polymer	149	16796.5	1	UniProt (P14119) Pfam (PF01280)	Haloarcula marismortui	Hl24,Hmal19
23	W (Q)	50S ribosomal protein L21e	polymer	96	10567.8	1	UniProt (P12734) Pfam (PF01157)	Haloarcula marismortui	Hl31
24	X (R)	50S ribosomal protein L22	polymer	155	16966.9	1	UniProt (P10970) Pfam (PF00237)	Haloarcula marismortui	Hl23,Hmal22
25	Y (S)	50S ribosomal protein L23	polymer	85	9612.5	1	UniProt (P12732) Pfam (PF00276)	Haloarcula marismortui	Hl25,Hmal23,L21
26	Z (T)	50S ribosomal protein L24	polymer	120	13671.0	1	UniProt (P10972) Pfam (PF16906) Pfam (PF00467)	Haloarcula marismortui	Hl15,Hl16,Hmal24
27	AA (U)	50S ribosomal protein L24e	polymer	66	7233.7	1	UniProt (P14116) Pfam (PF01246)	Haloarcula marismortui	Hl21/Hl22
28	BA (V)	50S ribosomal protein L29	polymer	71	7889.9	1	UniProt (P10971) Pfam (PF00831)	Haloarcula marismortui	Hl33,Hmal29
29	CA (W)	50S ribosomal protein L30	polymer	154	17062.9	1	UniProt (P14121) Pfam (PF00327)	Haloarcula marismortui	Hl16,Hl20,Hmal30
30	DA (X)	50S ribosomal protein L31e	polymer	92	10384.6	1	UniProt (P18138) Pfam (PF01198)	Haloarcula marismortui	Hl30,L34
31	EA (Y)	50S ribosomal protein L32e	polymer	241	26455.7	1	UniProt (P12736) Pfam (PF14520) Pfam (PF01655)	Haloarcula marismortui	Hl5
32	FA (Z)	50S ribosomal protein L37Ae	polymer	83	9409.3	1	UniProt (P60619) Pfam (PF01780)	Haloarcula marismortui	Ribosomal protein L43e
33	AB, AC, AD, AE, BB... (0, A, Y, B, K...)	MAGNESIUM ION	non-polymer		24.3	117	Chemie (MG)
34	LE, ME (0)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
35	AF, AG, AH, AI, BF... (0, 9, A, C, H...)	SODIUM ION	non-polymer		23.0	86	Chemie (NA)
36	CJ, DI, FI, JH, JI... (Y, A, B, 0, J...)	CHLORIDE ION	non-polymer		35.5	22	Chemie (CL)
37	AJ, DJ, SI, TH, VH (U, Z, O, 1, 3)	CADMIUM ION	non-polymer		112.4	5	Chemie (CD)
38	AK, BK, CK, DK, EJ... (Q, R, S, T, 0...)	water	water		18.0	7810	Chemie (HOH)

Sequence modifications

0: 1 - 2922 (GenBank: 55229667)

PDB	External Database	Details
A 2099	G 2636308	conflict

C: 1 - 246 (UniProt: P12735)

PDB	External Database	Details
Leu 73	Gln 73	conflict

G: 1 - 348 (UniProt: P15825)

PDB	External Database	Details
Asp 248	Ala 248	conflict

K: 1 - 132 (UniProt: P22450)

PDB	External Database	Details
Leu 44	His 44	conflict

M: 0 - 194 (UniProt: P60618)

PDB	External Database	Details
Glu 13	Lys 14	conflict

Z: 11 - 92 (UniProt: P60619)

PDB	External Database	Details
Arg 10	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	32
	Total formula weight	1475351.8
Non-Polymers*	Number of molecules	232
	Total formula weight	6241.0
All*	Total formula weight	1481592.8

*Water molecules are not included.