1YJA
SUBTILISIN BPN' 8397+1 (E.C. 3.4.21.14) (MUTANT WITH MET 50 REPLACED BY PHE, ASN 76 REPLACED BY ASP, GLY 169 REPLACED BY ALA, GLN 206 REPLACED BY CYS, ASN 218 REPLACED BY SER AND LYS 256 REPLACED BY TYR) (M50F, N76D, G169A, Q206C, N218S, AND K256Y) IN 20% DIMETHYLFORMAMIDE
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | SUBTILISIN 8397+1 | polymer | 275 | 27581.5 | 1 | UniProt (P00782) Pfam (PF00082) In PDB | Bacillus amyloliquefaciens | SUBTILISIN |
| 2 | A | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 3 | water | water | 18.0 | 65 | Chemie (HOH) |
Sequence modifications
A: 1 - 275 (UniProt: P00782)
| PDB | External Database | Details |
|---|---|---|
| Phe 50 | Met 157 | engineered mutation |
| Asp 76 | Asn 183 | engineered mutation |
| Ala 169 | Gly 276 | engineered mutation |
| Cso 206 | Gln 313 | engineered mutation |
| Ser 218 | Asn 325 | engineered mutation |
| Tyr 256 | Lys 363 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27581.5 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 80.2 | |
| All* | Total formula weight | 27661.7 |
