1YJ9

Crystal Structure Of The Mutant 50S Ribosomal Subunit Of Haloarcula Marismortui Containing a three residue deletion in L22

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (0)	23S Ribosomal RNA	polymer	2922	946157.4	1	GenBank (55229667)	Haloarcula marismortui
2	B (9)	5S Ribosomal RNA	polymer	122	39303.4	1	GenBank (3377778)	Haloarcula marismortui
3	C (A)	50S ribosomal protein L2P	polymer	240	25354.7	1	UniProt (P20276) Pfam (PF00181) Pfam (PF03947)	Haloarcula marismortui
4	D (B)	50S ribosomal protein L3P	polymer	338	37396.4	1	GenBank (55378384) Pfam (PF00297) UniProt (by SIFTS) (P20279)	Haloarcula marismortui
5	E (C)	50S ribosomal protein L4E	polymer	246	26442.1	1	UniProt (P12735) Pfam (PF00573)	Haloarcula marismortui
6	F (D)	50S ribosomal protein L5P	polymer	177	19551.7	1	UniProt (P14124) Pfam (PF00281) Pfam (PF00673)	Haloarcula marismortui
7	G (E)	50S ribosomal protein L6P	polymer	178	19961.7	1	UniProt (P14135) Pfam (PF00347)	Haloarcula marismortui
8	H (F)	50S ribosomal protein L7AE	polymer	120	12791.9	1	UniProt (P12743) Pfam (PF01248)	Haloarcula marismortui
9	I (G)	ACIDIC RIBOSOMAL PROTEIN P0 HOMOLOG	polymer	348	37213.8	1	UniProt (P15825) Pfam (PF00466) Pfam (PF17777)	Haloarcula marismortui
10	J (H)	50S RIBOSOMAL PROTEIN L10E	polymer	177	19942.7	1	GenBank (55231756) Pfam (PF00252) UniProt (by SIFTS) (P60617)	Haloarcula marismortui
11	K (I)	50S RIBOSOMAL PROTEIN L11P	polymer	162	17097.5	1	GenBank (132647) Pfam (PF03946) Pfam (PF00298) UniProt (by SIFTS) (P14122)	Haloarcula marismortui
12	L (J)	50S ribosomal protein L13P	polymer	145	16249.2	1	UniProt (P29198) Pfam (PF00572)	Haloarcula marismortui
13	M (K)	50S ribosomal protein L14P	polymer	132	14191.2	1	UniProt (P22450) Pfam (PF00238)	Haloarcula marismortui
14	N (L)	50S ribosomal protein L15P	polymer	165	18005.4	1	UniProt (P12737) Pfam (PF00828)	Haloarcula marismortui
15	O (M)	50S Ribosomal Protein L15E	polymer	195	22275.1	1	GenBank (55231501) Pfam (PF00827) UniProt (by SIFTS) (P60618)	Haloarcula marismortui
16	P (N)	50S ribosomal protein L18P	polymer	187	20640.9	1	UniProt (P14123) Pfam (PF17144)	Haloarcula marismortui
17	Q (O)	50S ribosomal protein L18e	polymer	116	12438.9	1	UniProt (P12733) Pfam (PF00828)	Haloarcula marismortui
18	R (P)	50S ribosomal protein L19E	polymer	149	16796.5	1	UniProt (P14119) Pfam (PF01280)	Haloarcula marismortui
19	S (Q)	50S ribosomal protein L21e	polymer	96	10567.8	1	UniProt (P12734) Pfam (PF01157)	Haloarcula marismortui
20	T (R)	50S ribosomal protein L22P	polymer	152	16653.6	1	UniProt (P10970) Pfam (PF00237)	Haloarcula marismortui
21	U (S)	50S ribosomal protein L23P	polymer	85	9612.5	1	UniProt (P12732) Pfam (PF00276)	Haloarcula marismortui
22	V (T)	50S ribosomal protein L24P	polymer	120	13671.0	1	UniProt (P10972) Pfam (PF16906) Pfam (PF00467)	Haloarcula marismortui
23	W (U)	50S ribosomal protein L24E	polymer	66	7233.7	1	UniProt (P14116) Pfam (PF01246)	Haloarcula marismortui
24	X (V)	50S ribosomal protein L29P	polymer	71	7889.9	1	UniProt (P10971) Pfam (PF00831)	Haloarcula marismortui
25	Y (W)	50S ribosomal protein L30P	polymer	154	17062.9	1	UniProt (P14121) Pfam (PF00327)	Haloarcula marismortui
26	Z (X)	50S ribosomal protein L31e	polymer	92	10384.6	1	UniProt (P18138) Pfam (PF01198)	Haloarcula marismortui
27	AA (Y)	50S ribosomal protein L32E	polymer	241	26455.7	1	UniProt (P12736) Pfam (PF14520) Pfam (PF01655)	Haloarcula marismortui
28	BA (Z)	50S ribosomal protein L37Ae	polymer	83	9409.3	1	GenBank (55231162) Pfam (PF01780) UniProt (by SIFTS) (P60619)	Haloarcula marismortui
29	CA (1)	50S ribosomal protein L37e	polymer	57	6330.2	1	UniProt (P32410) Pfam (PF01907)	Haloarcula marismortui
30	DA (2)	50S ribosomal protein L39e	polymer	50	6133.1	1	UniProt (P22452) Pfam (PF00832)	Haloarcula marismortui
31	EA (3)	50S ribosomal protein L44E	polymer	92	10815.2	1	UniProt (P32411) Pfam (PF00935)	Haloarcula marismortui
32	AB, AC, AD, AE, BB... (0, K, 9, T, A...)	MAGNESIUM ION	non-polymer		24.3	116	Chemie (MG)
33	IE, JE (0)	POTASSIUM ION	non-polymer		39.1	2	Chemie (K)
34	AF, AG, AH, BF, BG... (0, L, M, Q, R...)	SODIUM ION	non-polymer		23.0	86	Chemie (NA)
35	AI, BI, BJ, CI, FI... (J, 3, L, 0, M...)	CHLORIDE ION	non-polymer		35.5	22	Chemie (CL)
36	AJ, JI, TI, WI, XI (3, O, U, Z, 1)	CADMIUM ION	non-polymer		112.4	5	Chemie (CD)
37	AK, BK, CJ, CK, DJ... (W, X, 0, Y, 9...)	water	water		18.0	7784	Chemie (HOH)

Sequence modifications

0: 1 - 2922 (GenBank: 55229667)

PDB	External Database	Details
1ma 628	A 627	modified residue
Omu 2587	U 2586	modified residue
Omg 2588	G 2587	modified residue
Ur3 2619	U 2618	modified residue
Psu 2621	U 2620	modified residue

K: 1 - 132 (UniProt: P22450)

PDB	External Database	Details
Leu 44	His 44	conflict

M: 0 - 194 (GenBank: 55231501)

PDB	External Database	Details
Glu 13	Lys 14	conflict

R: 0 - 151 (UniProt: P10970)

PDB	External Database	Details
-	Gln 122	deletion
-	Gln 123	deletion
-	Gly 124	deletion

Z: 10 - 92 (GenBank: 55231162)

PDB	External Database	Details
Arg 10	Ser 34	conflict

Contents of the asymmetric unit

Polymers	Number of chains	31
	Total formula weight	1474030.3
Non-Polymers*	Number of molecules	231
	Total formula weight	6216.7
All*	Total formula weight	1480247.0

*Water molecules are not included.