1Y3B
Crystal structure of the complex of subtilisin BPN' with chymotrypsin inhibitor 2 E60S mutant
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | E | subtilisin BPN' | polymer | 281 | 28381.4 | 1 | UniProt (P00782) Pfam (PF00082) In PDB | Bacillus amyloliquefaciens | Subtilisin Novo; Subtilisin DFE; Alkaline protease |
2 | I | chymotrypsin inhibitor 2 | polymer | 64 | 7241.6 | 1 | UniProt (Q40059) Pfam (PF00280) In PDB | Hordeum vulgare | |
3 | E | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
4 | E | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
5 | E | CITRIC ACID | non-polymer | 192.1 | 3 | Chemie (CIT) | |||
6 | E | POLYETHYLENE GLYCOL (N=34) | non-polymer | 1529.8 | 3 | Chemie (15P) | |||
7 | water | water | 18.0 | 504 | Chemie (HOH) |
Sequence modifications
E: 1 - 275 (UniProt: P00782)
I: 21 - 83 (UniProt: Q40059)
PDB | External Database | Details |
---|---|---|
His 276 | - | expression tag |
His 277 | - | expression tag |
His 278 | - | expression tag |
His 279 | - | expression tag |
His 280 | - | expression tag |
His 281 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met 20 | - | initiating methionine |
Ser 60 | Glu 61 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 35623.0 | |
Non-Polymers* | Number of molecules | 8 |
Total formula weight | 5228.9 | |
All* | Total formula weight | 40851.9 |