1XTF

neurotoxin BoNT/A E224Q Y366F mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	neurotoxin BoNT/A	polymer	427	48975.2	2	GenBank (33321098) Pfam (PF01742) UniProt (by SIFTS) (P0DPI1) In PDB	Clostridium botulinum
2	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3		water	water		18.0	313	Chemie (HOH)

Sequence modifications

A, B: 2 - 420 (GenBank: 33321098)

PDB	External Database	Details
Gln 224	Glu 224	engineered mutation
Phe 366	Tyr 366	engineered mutation
Pro 421	-	cloning artifact
Gly 422	-	cloning artifact
His 423	-	expression tag
His 424	-	expression tag
His 425	-	expression tag
His 426	-	expression tag
His 427	-	expression tag
His 428	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97950.4
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	98081.3

*Water molecules are not included.