1X1Y

Water-mediate interaction at aprotein-protein interface

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Ribonuclease	polymer	110	12341.7	3	UniProt (P00648) Pfam (PF00545) In PDB	Bacillus amyloliquefaciens	Barnase, RNase Ba
2	D, E, F	Barstar	polymer	90	10244.6	3	UniProt (P11540) Pfam (PF01337) In PDB	Bacillus amyloliquefaciens	Ribonuclease inhibitor
3		water	water		18.0	566	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 110 (UniProt: P00648)

PDB	External Database	Details
Ala 2	Gln 49	engineered mutation

D, E, F: 0 - 89 (UniProt: P11540)

PDB	External Database	Details
Ala 35	Asp 35	engineered mutation
Ala 40	Cys 40	engineered mutation
Ala 82	Cys 82	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	67758.8
All*	Total formula weight	67758.8

*Water molecules are not included.