1WZE

Structural basis for alteration of cofactor specificity of Malate dehydrogenase from Thermus flavus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Malate dehydrogenase	polymer	327	35460.6	2	UniProt (P10584) Pfam (PF00056) Pfam (PF02866) In PDB	Thermus thermophilus
2	A, B	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
3		water	water		18.0	438	Chemie (HOH)

Sequence modifications

A, B: 0 - 332 (UniProt: P10584)

PDB	External Database	Details
Gly 41	Glu 42	engineered mutation
Ser 42	Ile 43	engineered mutation
Glu 43	Pro 44	engineered mutation
Arg 44	Gln 45	engineered mutation
Ser 45	Ala 46	engineered mutation
Phe 46	Met 47	engineered mutation
Gln 47	Lys 48	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	70921.3
Non-Polymers*	Number of molecules	2
	Total formula weight	1326.8
All*	Total formula weight	72248.1

*Water molecules are not included.