1W5Q
Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations A129C, D131C, D139C, P132E, K229R)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DELTA-AMINOLEVULINIC ACID DEHYDRATASE | polymer | 337 | 37132.1 | 2 | UniProt (Q59643) Pfam (PF00490) | PSEUDOMONAS AERUGINOSA | PORPHOBILINOGEN SYNTHASE, ALAD, ALADHPORPHOBILINOGEN SYNTHASE |
| 2 | C, I (A, B) | FORMIC ACID | non-polymer | 46.0 | 2 | Chemie (FMT) | |||
| 3 | D, F, G, J, K (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 4 | E, L (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 5 | H, M (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 6 | N, O (A, B) | water | water | 18.0 | 619 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 337 (UniProt: Q59643)
| PDB | External Database | Details |
|---|---|---|
| Cys 129 | Ala 129 | engineered mutation |
| Cys 131 | Asp 131 | engineered mutation |
| Glu 132 | Pro 132 | engineered mutation |
| Cys 139 | Asp 139 | engineered mutation |
| Cys 139 | Asp 139 | engineered mutation |
| Val 199 | Ile 199 | SEE REMARK 999 |
| Arg 229 | Lys 229 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 74264.2 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 390.4 | |
| All* | Total formula weight | 74654.5 |
