1W5Q

Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations A129C, D131C, D139C, P132E, K229R)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	DELTA-AMINOLEVULINIC ACID DEHYDRATASE	polymer	337	37132.1	2	UniProt (Q59643) Pfam (PF00490) In PDB	PSEUDOMONAS AERUGINOSA	PORPHOBILINOGEN SYNTHASE, ALAD, ALADHPORPHOBILINOGEN SYNTHASE
2	A, B	FORMIC ACID	non-polymer		46.0	2	Chemie (FMT)
3	A, B	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
4	A, B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	A, B	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
6		water	water		18.0	619	Chemie (HOH)

Sequence modifications

A, B: 1 - 337 (UniProt: Q59643)

PDB	External Database	Details
Cys 129	Ala 129	engineered mutation
Cys 131	Asp 131	engineered mutation
Glu 132	Pro 132	engineered mutation
Cys 139	Asp 139	engineered mutation
Cys 139	Asp 139	engineered mutation
Val 199	Ile 199	SEE REMARK 999
Arg 229	Lys 229	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74264.2
Non-Polymers*	Number of molecules	11
	Total formula weight	390.4
All*	Total formula weight	74654.5

*Water molecules are not included.