1W5P
Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations A129C, D131C, D139C, P132E)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DELTA-AMINOLEVULINIC ACID DEHYDRATASE | polymer | 337 | 37104.1 | 2 | UniProt (Q59643) Pfam (PF00490) In PDB | PSEUDOMONAS AERUGINOSA | PORPHOBILINOGEN SYNTHASE, ALAD, ALADHPORPHOBILINOGEN SYNTHASE |
| 2 | A, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 3 | A, B | FORMIC ACID | non-polymer | 46.0 | 2 | Chemie (FMT) | |||
| 4 | A, B | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 5 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | A, B | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 7 | A, B | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 8 | A, B | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 9 | water | water | 18.0 | 531 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 337 (UniProt: Q59643)
| PDB | External Database | Details |
|---|---|---|
| Cys 129 | Ala 129 | engineered mutation |
| Cys 131 | Asp 131 | engineered mutation |
| Glu 132 | Pro 132 | engineered mutation |
| Cys 139 | Asp 139 | engineered mutation |
| Val 199 | Ile 199 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 74208.1 | |
| Non-Polymers* | Number of molecules | 21 |
| Total formula weight | 1404.7 | |
| All* | Total formula weight | 75612.8 |
