1W56
Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations A129C and D131C)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DELTA-AMINOLEVULINIC ACID DEHYDRATASE | polymer | 337 | 37084.0 | 2 | UniProt (Q59643) Pfam (PF00490) | PSEUDOMONAS AERUGINOSA | PORPHOBILINOGEN SYNTHASE, ALAD, ALADHPORPHOBILINOGEN SYNTHASE |
| 2 | C, G (A, B) | FORMIC ACID | non-polymer | 46.0 | 2 | Chemie (FMT) | |||
| 3 | D, H, I (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 4 | E, J (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 5 | F, K (A, B) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 6 | L, M (A, B) | water | water | 18.0 | 693 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 337 (UniProt: Q59643)
| PDB | External Database | Details |
|---|---|---|
| Cys 129 | Ala 129 | engineered mutation |
| Cys 131 | Asp 131 | engineered mutation |
| Val 199 | Ile 199 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 74168.0 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 374.0 | |
| All* | Total formula weight | 74542.0 |
