1V3M
Crystal structure of F283Y mutant cyclodextrin glycosyltransferase complexed with a pseudo-tetraose derived from acarbose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Cyclomaltodextrin glucanotransferase | polymer | 686 | 75246.1 | 2 | UniProt (P05618) Pfam (PF00128) Pfam (PF02806) Pfam (PF01833) Pfam (PF00686) In PDB | Bacillus sp. | Cyclodextrin-glycosyltransferase, CGTase |
| 2 | C, D | 4,6-dideoxy-alpha-D-xylo-hexopyranose-(1-4)-beta-D-galactopyranose | branched | 310.3 | 2 | In PDB GlyTouCan (G94251DX) | |||
| 3 | E | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | A, B | alpha-D-glucopyranose | non-polymer | 180.2 | 4 | Chemie (GLC) | |||
| 5 | A, B | 6-AMINO-4-HYDROXYMETHYL-CYCLOHEX-4-ENE-1,2,3-TRIOL | non-polymer | 175.2 | 2 | Chemie (ACI) | |||
| 6 | A, B | beta-D-galactopyranose | non-polymer | 180.2 | 3 | Chemie (GAL) | |||
| 7 | A, B | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 8 | water | water | 18.0 | 685 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 686 (UniProt: P05618)
| PDB | External Database | Details |
|---|---|---|
| Tyr 283 | Phe 310 | engineered mutation |
| Pro 452 | Arg 479 | SEE REMARK 999 |
| Gly 454 | Ala 481 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 150492.2 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 962.9 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1771.8 | |
| All* | Total formula weight | 153226.8 |
