1V3I
The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Beta-amylase | polymer | 495 | 55964.2 | 1 | UniProt (P10538) Pfam (PF01373) In PDB | Glycine max (soybean) | 1,4-alpha-D-glucan maltohydrolase |
| 2 | B | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 3 | C | alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900018) GlyTouCan (G66120GK) | beta-maltose | ||
| 4 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 5 | water | water | 18.0 | 419 | Chemie (HOH) |
Sequence modifications
A: 1 - 495 (UniProt: P10538)
| PDB | External Database | Details |
|---|---|---|
| Leu 76 | Phe 76 | engineered mutation |
| Gly 202 | Arg 202 | SEE REMARK 999 |
| Gln 380 | Glu 380 | engineered mutation |
| Arg 399 | Lys 399 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 55964.2 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 96.1 | |
| All* | Total formula weight | 56744.8 |
