1UKS
Crystal structure of F183L/F259L mutant cyclodextrin glucanotransferase complexed with a pseudo-maltotetraose derived from acarbose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Cyclomaltodextrin glucanotransferase | polymer | 686 | 75162.1 | 2 | UniProt (P05618) Pfam (PF00128) Pfam (PF02806) Pfam (PF01833) Pfam (PF00686) In PDB | Bacillus sp. | Cyclodextrin-glycosyltransferase, CGTase, CYCLODEXTRIN GLUCANOTRANSFERASE |
| 2 | C, D | 4,6-dideoxy-alpha-D-xylo-hexopyranose-(1-4)-beta-D-galactopyranose | branched | 310.3 | 2 | In PDB GlyTouCan (G94251DX) | |||
| 3 | A, B | alpha-D-glucopyranose | non-polymer | 180.2 | 2 | Chemie (GLC) | |||
| 4 | A, B | 6-AMINO-4-HYDROXYMETHYL-CYCLOHEX-4-ENE-1,2,3-TRIOL | non-polymer | 175.2 | 2 | Chemie (ACI) | |||
| 5 | A, B | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 6 | water | water | 18.0 | 739 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 686 (UniProt: P05618)
| PDB | External Database | Details |
|---|---|---|
| Leu 183 | Phe 210 | engineered mutation |
| Leu 259 | Phe 286 | engineered mutation |
| Pro 452 | Arg 479 | SEE REMARK 999 |
| Gly 454 | Ala 481 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 150324.1 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 620.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 871.0 | |
| All* | Total formula weight | 151815.7 |
