1U71
Understanding the Role of Leu22 Variants in Methotrexate Resistance: Comparison of Wild-type and Leu22Arg Variant Mouse and Human Dihydrofolate Reductase Ternary Crystal Complexes with Methotrexate and NADPH
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Dihydrofolate reductase | polymer | 186 | 21393.6 | 1 | UniProt (P00374) Pfam (PF00186) | Homo sapiens (human) | |
| 2 | B, C (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 3 | D (A) | 6-(2,5-DIMETHOXY-BENZYL)-5-METHYL-PYRIDO[2,3-D]PYRIMIDINE-2,4-DIAMINE | non-polymer | 325.4 | 1 | Chemie (MXA) | |||
| 4 | E (A) | water | water | 18.0 | 68 | Chemie (HOH) |
Sequence modifications
A: 1 - 186 (UniProt: P00374)
| PDB | External Database | Details |
|---|---|---|
| Arg 22 | Leu 22 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 21393.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 517.5 | |
| All* | Total formula weight | 21911.1 |
