1U0D

Y33H Mutant of Homing endonuclease I-CreI

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	5'-D(*GP*CP*GP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3'	polymer	24	7419.8	1
2	B (D)	5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*CP*GP*C)-3'	polymer	24	7321.7	1
3	C, D (A, B)	DNA endonuclease I-CreI	polymer	163	18698.5	2	UniProt (P05725) Pfam (PF00961)	Chlamydomonas reinhardtii	23S rRNA intron protein

Sequence modifications

A, B: 1 - 163 (UniProt: P05725)

PDB	External Database	Details
His 33	Tyr 33	engineered mutation
Thr 42	Ala 42	engineered mutation
Glu 47	Gln 47	engineered mutation
Glu 110	Trp 110	engineered mutation
Gln 111	Arg 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52138.5
All*	Total formula weight	52138.5

*Water molecules are not included.