1TW1

beta-1,4-galactosyltransferase mutant Met344His (m344H-Gal-T1) complex with UDP-galactose and magnesium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-1,4-galactosyltransferase 1	polymer	286	32856.5	2	UniProt (P08037) Pfam (PF13733) Pfam (PF02709)	Bos taurus (cattle)	Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1
2	C, I (A, B)	GALACTOSE-URIDINE-5'-DIPHOSPHATE	non-polymer		566.3	2	Chemie (GDU)
3	D, J (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E, F, K, L, M (A, B)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
5	G, H (A)	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	2	Chemie (DIO)
6	N (B)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
7	O, P (A, B)	water	water		18.0	522	Chemie (HOH)

Sequence modifications

A, B: 130 - 402 (UniProt: P08037)

PDB	External Database	Details
Ala 117	-	SEE REMARK 999
Ser 118	-	SEE REMARK 999
Met 119	-	SEE REMARK 999
Thr 120	-	SEE REMARK 999
Gly 121	-	SEE REMARK 999
Gly 122	-	SEE REMARK 999
Gln 123	-	SEE REMARK 999
Gln 124	-	SEE REMARK 999
Met 125	-	SEE REMARK 999
Gly 126	-	SEE REMARK 999
Arg 127	-	SEE REMARK 999
Gly 128	-	SEE REMARK 999
Ser 129	-	SEE REMARK 999
Thr 342	Cys 269	engineered mutation
His 344	Met 271	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65713.1
Non-Polymers*	Number of molecules	12
	Total formula weight	2033.0
All*	Total formula weight	67746.0

*Water molecules are not included.