1TMH
MODULAR MUTAGENESIS OF A TIM-BARREL ENZYME: THE CRYSTAL STRUCTURE OF A CHIMERIC E. COLI TIM HAVING THE EIGHTH (BETA-ALPHA)-UNIT REPLACED BY THE EQUIVALENT UNIT OF CHICKEN TIM
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | TRIOSEPHOSPHATE ISOMERASE | polymer | 254 | 27118.8 | 4 | UniProt (P0A858) Pfam (PF00121) UniProt (by SIFTS) (P00940) | Escherichia coli | |
| 2 | E, F, G, H (A, B, C, D) | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) |
Sequence modifications
A, B, C, D: 3 - 257 (UniProt: P0A858)
| PDB | External Database | Details |
|---|---|---|
| His 227 | Pro 225 | engineered mutation |
| Val 229 | Ile 227 | engineered mutation |
| Phe 232 | Ala 230 | engineered mutation |
| Pro 241 | Ala 239 | engineered mutation |
| - | Asp 240 | deletion |
| Glu 243 | Ala 241 | engineered mutation |
| Val 245 | Ala 243 | engineered mutation |
| Asp 246 | Val 244 | engineered mutation |
| Ile 248 | Val 246 | engineered mutation |
| Asn 249 | Lys 247 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 108475.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 384.3 | |
| All* | Total formula weight | 108859.6 |
