1TEF
Crystal structure of the spinach plastocyanin mutants G8D/K30C/T69C and K30C/T69C- a study of the effect on crystal packing and thermostability from the introduction of a novel disulfide bond
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Plastocyanin, chloroplast | polymer | 99 | 10454.6 | 2 | UniProt (P00289) Pfam (PF00127) In PDB | Spinacia oleracea (spinach) | |
2 | A, B | COPPER (II) ION | non-polymer | 63.5 | 2 | Chemie (CU) | |||
3 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
4 | water | water | 18.0 | 199 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 99 (UniProt: P00289)
PDB | External Database | Details |
---|---|---|
Asp 8 | Gly 77 | engineered mutation |
Cys 30 | Lys 99 | engineered mutation |
Cys 69 | Thr 138 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 20909.2 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 162.5 | |
All* | Total formula weight | 21071.7 |