1T9I

I-CreI(D20N)/DNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	5'-D(*GP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*TP*CP*G)-3'	polymer	24	7418.8	1
2	B (D)	5'-D(*CP*GP*AP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP*GP*C)-3'	polymer	24	7320.7	1
3	C, D (A, B)	DNA endonuclease I-CreI	polymer	163	18721.6	2	UniProt (P05725) Pfam (PF00961)	Chlamydomonas reinhardtii	23S rRNA intron protein
4	E, F (C)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	G, H, I, J (C, D, A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
6	K, L, M, N (C, D, A, B)	water	water		18.0	656	Chemie (HOH)

Sequence modifications

A, B: 1 - 163 (UniProt: P05725)

PDB	External Database	Details
Asn 20	Asp 20	engineered mutation
Thr 42	Ala 42	SEE REMARK 999
Glu 110	Trp 110	SEE REMARK 999
Gln 111	Arg 111	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52182.7
Non-Polymers*	Number of molecules	6
	Total formula weight	172.1
All*	Total formula weight	52354.8

*Water molecules are not included.