1T7Q

Crystal structure of the F565A mutant of murine carnitine acetyltransferase in complex with carnitine and CoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Carnitine acetyltransferase	polymer	618	70021.1	2	UniProt (P47934) Pfam (PF00755)	Mus musculus (house mouse)	CRAT
2	C, F (A, B)	COENZYME A	non-polymer		767.5	2	Chemie (COA)
3	D, G (A, B)	CARNITINE	non-polymer		162.2	2	Chemie (152)
4	E, H (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	I, J (A, B)	water	water		18.0	1148	Chemie (HOH)

Sequence modifications

A, B: 30 - 626 (UniProt: P47934)

PDB	External Database	Details
Met 9	-	expression tag
Gly 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
Ser 19	-	expression tag
Ser 20	-	expression tag
Gly 21	-	expression tag
Leu 22	-	expression tag
Val 23	-	expression tag
Pro 24	-	expression tag
Arg 25	-	expression tag
Gly 26	-	expression tag
Ser 27	-	expression tag
His 28	-	expression tag
Met 29	-	expression tag
Ala 565	Phe 565	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	140042.2
Non-Polymers*	Number of molecules	6
	Total formula weight	1983.6
All*	Total formula weight	142025.8

*Water molecules are not included.