1T2N
Structure of a thermostable triple mutant of Bacillus subtilis lipase obtained through directed evolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Lipase | polymer | 181 | 19459.9 | 1 | UniProt (P37957) Pfam (PF01674) | Bacillus subtilis | Triacylglycerol lipase |
| 2 | B (A) | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
| 3 | C (A) | water | water | 18.0 | 192 | Chemie (HOH) |
Sequence modifications
A: 1 - 181 (UniProt: P37957)
| PDB | External Database | Details |
|---|---|---|
| Pro 114 | Leu 145 | engineered mutation |
| Asp 132 | Ala 163 | engineered mutation |
| Tyr 166 | Asn 197 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 19459.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 39.1 | |
| All* | Total formula weight | 19499.0 |
