1S4P
Crystal structure of yeast alpha1,2-mannosyltransferase Kre2p/Mnt1p: ternary complex with GDP/Mn and methyl-alpha-mannoside acceptor
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Glycolipid 2-alpha-mannosyltransferase | polymer | 348 | 41349.2 | 2 | UniProt (P27809) Pfam (PF01793) | Saccharomyces cerevisiae (baker's yeast) | Alpha-1,2-mannosyltransferase |
| 2 | C (C) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1397.2 | 1 | In PDB GlyTouCan (G80966KZ) | |||
| 3 | D (D) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 4 | E, K (A, B) | methyl alpha-D-mannopyranoside | non-polymer | 194.2 | 2 | Chemie (MMA) | |||
| 5 | F, L (A, B) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 6 | G, H, M, N (A, B) | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
| 7 | I, O (A, B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 8 | J, P (A, B) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 2 | Chemie (EPE) | |||
| 9 | Q, R (A, B) | water | water | 18.0 | 853 | Chemie (HOH) |
Sequence modifications
A, B: 97 - 442 (UniProt: P27809)
| PDB | External Database | Details |
|---|---|---|
| Glu 95 | - | cloning artifact |
| Phe 96 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 82698.4 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1821.6 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2003.1 | |
| All* | Total formula weight | 86523.1 |
